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KMID : 0381120100320030225
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Genes and Genomics
2010 Volume.32 No. 3 p.225 ~ p.231
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The frequency of neural stem cells in in vitro culture systems: insights from simple modeling
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Choi Sun-Shim
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Abstract
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Neural stem cell (NSC) culture offers a renewable resource for cell replacement treatment of neurodegenerative diseases. In a NSC culture, the frequency of NSCs is estimated to be only a few percent, at most, while the majority of cells are neuroprogenitor cells (NPCs), the progeny of NSCs with a limited capability to proliferate. Here, the effects of several cell culture parameters on the cell composition of NSC cultures were illustrated over time using a simple model. The model shows thatvarious initial cell compositions in NSC cultures converge into a single steady state. The model also implies that the rate of increase in total cell number entirely depends on the self-renewal rate of NSCs, regardless of the proliferative capacity of NPCs. Furthermore, the model predicts that difference sin cell passage methods between neurosphere and monolayer cultures results in a change in the frequency of NSCs in culture.
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KEYWORD
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Neuroprogenitor, neurosphere, adherent monolayer culture, self-renewal, proliferation
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